Cell Line Lysates
Rockland Immunochemicals PC-3 Whole Cell Lysate 500µg
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µL depending on the size format of your gel.
Sino Biological Recombinant Human TRPM7 Protein (GST Tag), Active
Recombinant human CHAK1 (TRPM7) (1158-1865) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Sino Biological Recombinant Cynomolgus LDLR/LDL Receptor Protein (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified
A DNA sequence encoding the Cynomolgus LDLR (XP_005588053.3) (Met1-Gly788) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Sino Biological Recombinant Human CNR1 Protein (Nanodisc, Flag-His Tag)
A DNA sequence encoding the Human CNR1 (P21554-1)(Asp74-Ala305) & (Asp333-Ser414) was expressed and linked by a Fusion Protein, with a Flag tag followed by a polyhistidine tag at the N-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.
Sino Biological Recombinant Human CCR7/CD197 Protein (VLP, Flag Tag)
A DNA sequence encoding the Human CCR7-VLP (Full Length) (P32248) (Met1-Leu344, thermostabilizing mutations) was fused with a flag tag at the C-terminus. This protein was prepared using our VLP platform.As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Macherey-Nagel MACHEREY-NAGEL Lysis Buffer LYS-EF, Endotoxin-Free Plasmid Isolation, Compatible With NucleoBond Xtra Kits, 1000 mL
The MACHEREY-NAGEL Lysis Buffer LYS-EF is designed for effective cell lysis in endotoxin-free plasmid DNA isolation, essential for high-quality DNA suitable for sensitive molecular biology applications. This buffer is particularly compatible with NucleoBond Xtra Midi and Maxi EF kits, setting it apart for its specialized formulation that meets rigorous research standards.
Sino Biological Recombinant Mouse Cd1d1 & B2M Heterodimer Protein (His Tag), Endotoxin-Free
A DNA sequence encoding Mouse Cd1d1 (NP_031665.2) (Met1-Gly305) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Mouse B2M (P01887) (Met1-Met119, A105D) constructed the plasmid 2. The two plasmids were co-expressed and the Mouse Cd1d1/B2M heterodimer was purified.
Sino Biological Recombinant Human CCR7/CD197 Protein (VLP, Flag Tag)
A DNA sequence encoding the Human CCR7-VLP (Full Length) (P32248) (Met1-Leu344, thermostabilizing mutations) was fused with a flag tag at the C-terminus. This protein was prepared using our VLP platform.As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Revvity Health Sciences Inc HTRF Human and Mouse Total Cereblon Detection Kit, Control Lysate
HTRF Human and Mouse Total Cereblon Detection Kit, Control Lysate
Sino Biological Recombinant Human CNR1 Protein (Nanodisc, Flag-His Tag)
A DNA sequence encoding the Human CNR1 (P21554-1)(Asp74-Ala305) & (Asp333-Ser414) was expressed and linked by a Fusion Protein, with a Flag tag followed by a polyhistidine tag at the N-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.
Cayman Chemical 7DeazaadenosIn5OtrIphos 2.5mg
A modified nucleotide and an active metabolite of tubercidin; incorporated into the DNA of mouse L-cells using DNA polymerase α but not into the RNA of E. coli using RdRps; has been used as a probe for the ATP binding site of PKA
STEMCELL Technologies Human Platelet Lysate, Fibrinogen-Depleted, Size: 100 mL
Human platelet lysate is a growth factor-rich cell culture supplement derived from healthy donor human platelets at U.S. Food and Drug Administration (FDA)-licensed blood centers. Multiple donor units are pooled during manufacturing to minimize lot-to-lot variability. Pharmaceutical-grade heparin derived from porcine intestine is used in the manufacturing process. The concentration of heparin in the final product is ≤ 2 IU/mL.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.